R&D Experiment: WLP655 Belgian Sour Mix 1 Over Time

WLP655 Belgian Sour Mix 1 is a unique blend of Saccharomyces, Brettanomyces, Lactobacillus, and Pediococcus. Beers produced with Belgian blends, such as WLP655, are often aged in bottles or barrels for several months, continuously evolving their sensory profiles.

The impacts of different fermentation conditions on beer evolution are not fully understood. Our R&D team experimented with different pitch rates of WLP655 and its impact on beer fermentation and sensory evolution during cellaring. 

WLP655 Yeast ParametersBrew With WLP655 Belgian Sour Mix 1

Wort was aseptically collected from White Labs Brewing Co. and pitched with WLP655 at 2 different pitch rates.

  • 1 Homebrew PurePitch® Next Generation per 5 gallons 
  • 2 Homebrew PurePitch® Next Generation per 5 gallons
Starting Plato was at 13 Plato. They were fermented for 9 days at 77°F/25°C. The carboys were then transferred to room temperature and cellared for 7 months. Samples were pulled monthly for analytical and sensory analysis. Acetic acid testing was also performed in months 5-7. Beers finished at 2 Plato.

Main Takeaways:

WLP655 RD ExperimentWLP655 PurePitch Next Generation Homebrew

Figure 1 (Left) WLP655 Homebrew PurePitch® Next Generation. (Right) R&D Fermentations


Sensory:
  • In most sensory months, the lower pitch rate was liked more and preferred over the higher pitch rate based on the overall balance of the beer. The higher pitch rate led to more sour intensity.
  • Slight sourness was first noted in the higher pitch rate after 3 months of cellaring, and slight sourness was noted in the lower pitch rate after 4 months of cellaring.
  • Best sensory between 5 and 6 months at the lower pitch rate (nice sourness and was more complex and had better mouthfeel than the higher pitch rate)
  • The higher dosage rate started to have too much acetic acid around 6 months, lower dosage rate had too much acetic acid around 7 months.

Pellicle Formation after 4 months

RDExperimentWLP655 Belgian Sour Mix 1 Over TimeTotal Acidity Over Time

Total Acidity WLP655

Acetic Acid Over Time

WLP655 Acetic AcidFor this experiment in sensory trials, the higher dosage rate started to have too much acetic acid around 6 months, lower dosage rate had too much acetic acid around 7 months.

Read Our Blog About pH vs Total Acidity
Test for Beer Total Acidity
Test For Alt Beverage Titratable Acidity

Recent Posts

Featured image: Why Pitch Rate Matters (And How to Nail It Every Time)
Pitch Rate
Nov 18, 2025

Why Pitch Rate Matters (And How to Nail It Every Time)

Pitch rate is one of the most underrated levers in brewing, and yet it impacts everything from tank turns to ester profile to repitching health. When pitch rate fluctuates, so do fermentation speed, flavor, and your cellar schedule. Brewers can control multiple factors on the cold side. There are 3 key variables brewers can dial…

Read more
Featured image: What is the Next Generation of Pitching?
PurePitch Next Generation
Oct 28, 2025

What is the Next Generation of Pitching?

For decades, brewers have worked around yeast limitations, recycling from cone to cone, managing variability, and adjusting expectations. But yeast is alive, and even small shifts can cause significant differences in fermentation. That's where technology meets tradition. Over our 30 years of business, we have tinkered with the best ways to provide the best yeast…

Read more
Featured image: Tips From The Pitch: Winter is Coming, Big Beer Brewing
High Gravity Brewing
Tips From The Pitch
Oct 10, 2025

Tips From The Pitch: Winter is Coming, Big Beer Brewing

With winter on its way, now is the perfect time to craft robust beers that stand up to the season from barleywines and imperial stouts to other high-gravity styles. Get a head start on your brewing schedule to ensure these complex beers are perfectly aged and ready to enjoy by winter’s arrival. Making these big,…

Read more